LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2189..2285) /label=attL2 /note="attL2 (c)" misc_signal complement(2300..2319) /note="T7 Promoter/priming site (c)" primer_bind 2327..2343 /note="M13 Reverse priming site" gene 2456..3265 /note="Kanamycin resistance gene" rep_origin 3386..4059 /note="pUC origin" PCR_frame 669..2189 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2209) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2206) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2197..4062,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2167..2187 /note="TEV site" source 673..2166 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:5302 IMAGE:3456869" /tissue_type="Cervix, carcinoma" /clone_lib="NIH_MGC_12" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2166 /gene="KDELC1" /gene_synonym=EP58 /gene_synonym=KDEL1 /gene_synonym=MGC5302 /db_xref="GeneID:79070" /db_xref="HGNC:19350" CDS 673..2166 /dnas_title="KDEL (Lys-Asp-Glu-Leu) containing 1" /gene="KDELC1" /gene_synonym=EP58 /gene_synonym=KDEL1 /gene_synonym=MGC5302 /codon_start=1 /product="KDEL (Lys-Asp-Glu-Leu) containing 1" /protein_id="AAH01297.1" /db_xref="GI:12654903" /db_xref="GeneID:79070" /db_xref="HGNC:19350" /translation="MFGTLLLYCFFLATVPALAETGGERQLSPEKSEIWGPGLKADVV LPARYFYIQAVDTSGNKFTSSPGEKVFQVKVSAPEEQFTRVGVQVLDRKDGSFIVRYR MYASYKNLKVEVKFQGQHVAKSPYILKGPVYHENCDCPLQDSAAWLREMNCPETIAQI QRDLAHFPAVDPEKIAVEIPKRFGQRQSLCHYTLKDNKVYIKTHGEHVGFRIFMDAIL LSLTRKVKMPDVELFVNLGDWPLEKKKSNSNIHPIFSWCGSTDSKDIVMPTYDLTDSV LETMGRVSLDMMSVQANTGPPWESKNSTAVWRGRDSRKERLELVKLSRKHPELIDAAF TNFFFFKHDENLYGPIVKHISFFDFFKHKYQINIDGTVAAYRLPYLLVGDSVVLKQDS IYYEHFYNELQPWKHYIPVKSNLSDLLEKLKWAKDHDEEAKKIAKAGQEFARNNLMGD DIFCYYFKLFQEYANLQVSEPQIREGMKRVEPQTEDDLFPCTCHRKKTKDEL" misc_difference 1012..1012 /gene="KDELC1" /gene_synonym=EP58 /gene_synonym=KDEL1 /gene_synonym=MGC5302 /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 673..2166 /note="KDELC1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgtttgg cactttgcta ctttattgct tctttctggc gacagttcca 721 gcactcgccg agaccggcgg agaaaggcag ctgagcccgg agaagagcga aatatgggga 781 cccgggctaa aagcagacgt cgtccttccc gcccgctatt tctatattca ggcagtggat 841 acatcaggga ataaattcac atcttctcca ggcgaaaagg tcttccaggt gaaagtctca 901 gcaccagagg agcaattcac tagagttgga gtccaggttt tagaccgaaa agatgggtcc 961 ttcatagtaa gatacagaat gtatgcaagc tacaaaaatc tgaaggtgga agttaaattc 1021 caagggcaac atgtggccaa atccccatat attttaaaag ggccggttta ccatgagaac 1081 tgtgactgtc ctctgcaaga tagtgcagcc tggctacggg agatgaactg ccctgaaacc 1141 attgctcaga ttcagagaga tctggcacat ttccctgctg tggatccaga aaagattgca 1201 gtagaaatcc caaaaagatt tggacagagg cagagcctat gtcactacac cttaaaggat 1261 aacaaggttt atatcaagac tcatggtgaa catgtaggtt ttagaatttt catggatgcc 1321 atactacttt ctttgactag aaaggtgaag atgccagatg tggagctctt tgttaatttg 1381 ggagactggc ctttggaaaa aaagaaatcc aattcaaaca tccatccgat cttttcctgg 1441 tgtggctcca cagattccaa ggatatcgtg atgcctacgt acgatttgac tgattctgtt 1501 ctggaaacca tgggccgggt aagtctggat atgatgtccg tgcaagctaa cacgggtcct 1561 ccctgggaaa gcaaaaattc cactgccgtc tggagagggc gagacagccg caaagagaga 1621 ctcgagctgg ttaaactcag tagaaaacac ccagaactca tagacgctgc tttcaccaac 1681 tttttcttct ttaaacacga tgaaaacctg tatggtccca ttgtgaaaca tatttcattt 1741 tttgatttct tcaagcataa gtatcaaata aatatcgatg gcactgtagc agcttatcgc 1801 ctgccatatt tgctagttgg tgacagtgtt gtgctgaagc aggattccat ctactatgaa 1861 catttttaca atgagctgca gccctggaaa cactacattc cagttaagag caacctgagc 1921 gatctgctag aaaaacttaa atgggcgaaa gatcacgatg aagaggccaa aaagatagca 1981 aaagcaggac aagaatttgc aagaaataat ctcatgggcg atgacatatt ctgttattat 2041 ttcaaacttt tccaggaata tgccaattta caagtgagtg agccccaaat ccgagagggc 2101 atgaaaaggg tagaaccaca gactgaggac gacctcttcc cttgtacttg ccataggaaa 2161 aagaccgaaa acttgtactt tcaaggctCC AGCTTTCTTG TACAAAGTTG GCATTATAAG 2221 AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT 2281 TATTTGCCAT CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC 2341 CTGGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT 2401 ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG 2461 CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 2521 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGCTTGTA 2581 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 2641 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 2701 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGAAA 2761 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 2821 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 2881 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 2941 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 3001 ACTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 3061 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 3121 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 3181 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 3241 TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG 3301 CATTACGCTG ACTTGACGGG ACGGCGCAAG CTCATGACCA AAATCCCTTA ACGTGAGTTA 3361 CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC 3421 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT 3481 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC 3541 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC 3601 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 3661 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG 3721 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA 3781 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC 3841 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG 3901 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 3961 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT 4021 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TT //